Cyanoacrylate adhesives for reptile integument

[jitajide]

Hi all.

I was hoping for some advice concerning cyanoacrylate tissue adhesives.

I am currently working in Cuc Phuong national park, turtle conservation center in Vietnam as a veterinary volunteer.

We are planning to PIT tag 220 Mauremys annamensis semi-aquatic turtles for transfer to a release center in central vietnam.

I am unsure about which tissue adhesive to use to close the skin after implantation of the microchip. We need to order the product from the USA as not available here in Vietnam, and we are also concerned about the cost as of course this is a conservation center and therefore has limited funds, the PIT tags have been donated.



I have read extensively about tissumend II, 3M vetbond, Nexaband SC, durmabond etc... but cannot access info specifically concerning chelonia skin.
Individual doses will not be very cost effective, how much glue will be needed to close 220 or more microchip incision sites? how many animals can be treated with 3ml or 1,5 ml vials? containers?

Could anyone offer some more specific advice, as the ordering of these products is urgent.

If anyone has access to a good diagram showing the exact implant location site in the left hind limb it would be very much appreciated.

thanks in advance, hoping that this post in in the right place.

Dr Katherine Morgan

[Danchou]

With the support of the article below, say yes.

http://www.ncbi.nlm.nih.gov/pubmed/21962284

http://avmajournals.avma.org/doi/abs...r_pub%3dpubmed

"Am J Vet Res. 2011 Oct;72(10):1397-406.
Evaluation of the histologic reactions to commonly used suture materials in the skin and musculature of ball pythons (Python regius).

McFadden MS, Bennett RA, Kinsel MJ, Mitchell MA.
Source

Veterinary Teaching Hospital, Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA. mikemdvm@illinois.edu

Abstract

OBJECTIVE:

To evaluate histologic reactions to 8 suture materials and cyanoacrylate tissue adhesive (CTA) in the musculature and skin of ball pythons.
ANIMALS:

30 hatchling ball pythons.
PROCEDURES:

In each snake, ten 1-cm skin incisions were made (day 0). At 8 sites, a suture of 1 of 8 materials was placed in the epaxial musculature, and the incision was closed with the same material. One incision was closed by use of CTA. No suture material was placed in the tenth incision, which was allowed to heal by second intention (negative control). Snakes (n = 5/group) were euthanized for harvest of treatment-site tissues at days 3, 7, 14, 30, 60, and 90. Skin and muscle sections were examined microscopically and assigned a subjective score (0 to 4) for each of the following: overall severity of inflammation, fibrosis, number of macrophages, number of granulocytes, number of perivascular lymphocytes, and degree of suture fragmentation.
RESULTS:

Subjective score analysis revealed that CTA did not cause a significant inflammatory response, compared with the negative control. All suture materials caused significantly more inflammation over all time points; for all suture materials, inflammatory response scores were significantly higher than values for the negative control 90 days after implantation. No sutures were completely absorbed by the end of the study period, and several sutures appeared to be in the process of extrusion.
CONCLUSIONS AND CLINICAL RELEVANCE:

In snakes, CTA can be used to close small superficial incisions or lacerations with minimal inflammatory response, and sutures may undergo extrusion from tissues prior to complete absorption."