Methods of pregnancy diagnosis in the cow
Method .................................................. ...........................Earliest time
♦Early pregnancy factor (EPF)/early conception factor (ECF)>>>> 3 days
♦Realtime ultrasound (direct imaging)>>>>>> 13 days
♦Failure to return to oestrus and persistence of corpus luteum>>> 21 days
♦Progesterone concentration in plasma and milk >>>>21–24 days
♦Assay of pregnancy-specific protein B (PSPB)>>>>> 24 days
♦Palpation of the allantochorion (membrane slip) >>>>>>33 days
♦Unilateral cornual enlargement and disparity in size, thinning of the uterine wall, fluid-
filled fluctuation of enlarged horns >>>>>35 days
♦Palpation of the early fetus when the amnion loses its turgidity>>>>> 45–60 days
♦Palpation of the carnucles/cotyledons>>>>>>>>> 80 days
♦Hypertrophy of the middle uterine artery until presence of fremitus>>> 85 days
♦Oestrone sulphate in blood or milk>>>>>>>> 105 days
♦Palpation of the fetus>>>>>>>>>> 120 days



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